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KMID : 0379120150430040267
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Korean Journal of Mycology
2015 Volume.43 No. 4 p.267 ~ p.271
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Isolation and Identification of Yeasts from Jeju Island Soils
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Han Sang-Min
Bae Sang-Min
Han Jae-Won
Kim Ji-Yoon
Lee Jong-Soo
Kim Ha-Kun
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Abstract
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Significant differences in annual precipitates on Jeju island have been reported depending on the location. We collected soil samples from east and west areas of Jeju Island to identify yeasts by plating on yeast peptone dextrose plates and subsequent analysis for the polymerase chain reaction amplified D1/D2 region of 26S rDNA of colonies. As a result, 20 yeast strains of 12 species were isolated from 7 different sampling sites in east area and 13 yeast strains of 6 species from 5 different sampling sites in west area. Some differences in yeast flora were observed depending on the sample collection sites having different annual precipitates.
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KEYWORD
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D1/D2 region of 26S rDNA, Jeju Island soil samples, Yeast identification
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